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Median Normalization

normalize_median.Rd

Normalize the expression matrix by dividing each column (sample) by the median of that column (NA ignored), so that the median of each column is 1. This is the most common normalization method for proteomics data. It effectively and robustly removes the bias introduced by total protein abundance, and removes batch effects in part.

Usage

normalize_median(x)

Arguments

x

Either a glyexp_experiment object or a matrix. If a matrix, rows should be variables and columns should be samples.

Value

Returns the same type as the input. If x is a glyexp_experiment, returns a glyexp_experiment with normalized expression matrix. If x is a matrix, returns a normalized matrix.