Ion-source dopant effects on O-glycan signal

Targeted peak areas for 15 selected O-glycan features measured in technical injection replicates under six ion-source gas/dopant conditions.

Usage

glyhub_GPST000170_O(
  kind = c("curated", "raw"),
  cache_dir = getOption("glyhub.cache_dir", tools::R_user_dir("glyhub", "cache")),
  refresh = FALSE,
  offline = FALSE
)

Format

A glyexp::GlycomicSE with 15 features and 18 observations. The abundance assay is in peak area. rowData has no glycan_structure column because no feature has a supported structural annotation.

Source

GlycoPOST GPST000170. S1. Study: https://doi.org/10.1021/acs.analchem.1c00023. Repository: https://glycopost.glycosmos.org/entry/GPST000170.

Arguments

kind: "curated" returns a data object; "raw" returns the path to a verified source archive. Raw archives are currently available for CPTAC.
cache_dir: Cache directory, defaulting to tools::R_user_dir("glyhub", "cache"). Set option glyhub.cache_dir to change the default.
refresh: Download the pinned resource again, even if cached.
offline: Prohibit downloads; require a verified local resource.

Details

The panel compares AIR, nitrogen and solvent-enriched nitrogen conditions using three injections per condition. This is the complete author-defined 15-feature targeted panel, not an unbiased whole-glycome survey. Isomer suffixes remain separate; Sg denotes Neu5Gc. Relative area summaries and signal-to-noise statistics are not mistaken for extra samples. The N-glycan sample is a 1:1 mixture released from human IgG and bovine fetuin; the O-glycan sample derives from bovine submaxillary mucin. Each run injected 1 microlitre.

Source molecular compositions retained without inferring glycosidic linkages.

Access

Use glyhub_load("glyhub_GPST000170_O") to load this dataset.