Platelet N-glycan isomer profiles in control and FUT8-knockout mice

Fifty N-glycan chromatographic features quantified in platelet lysates from three control and three FUT8-knockout mouse observations, supporting a study of platelet activation and thrombosis.

Usage

glyhub_GPST000596_N(
  kind = c("curated", "raw"),
  cache_dir = getOption("glyhub.cache_dir", tools::R_user_dir("glyhub", "cache")),
  refresh = FALSE,
  offline = FALSE
)

Format

A glyexp::GlycomicSE with 50 features and 6 observations. The abundance assay is in area under chromatographic curve. rowData has no glycan_structure column because no feature has a supported structural annotation.

Source

GlycoPOST GPST000596. . Study: https://doi.org/10.1161/atvbaha.126.324757. Repository: https://glycopost.glycosmos.org/entry/GPST000596. Quantitative source: https://glycopost.glycosmos.org/data/GPST000596.0/FUT8-KO_Manuscript_SupplementaryTable_Final.xlsx.

Arguments

kind: "curated" returns a data object; "raw" returns the path to a verified source archive. Raw archives are currently available for CPTAC.
cache_dir: Cache directory, defaulting to tools::R_user_dir("glyhub", "cache"). Set option glyhub.cache_dir to change the default.
refresh: Download the pinned resource again, even if cached.
offline: Prohibit downloads; require a verified local resource.

Details

The assay contains the six original area-under-curve columns C1–C3 and K1–K3. Isomer labels, retention times, precursor charge and mass, glycan class and author-assigned fucosyl linkage are retained. The separate normalized block in the source workbook contains fractions despite its percent heading; it is not duplicated as an independent dataset. Replicate biology beyond the author condition labels is not inferred.

Explicit total Hex, HexNAc, Fuc, NeuGc and NeuAc counts are used. Distinct chromatographic isomers remain distinct features. Core/outer-arm linkage information is retained as an author annotation and is not expanded into an unsupported complete glycan topology.

Access

Use glyhub_load("glyhub_GPST000596_N") to load this dataset.