Galactosylation of bovine fetuin N-glycans in an enzyme reactor

N-glycan profiles from bovine fetuin treated with an immobilised B4GalT1 reactor. The experiment examines galactose addition to desialylated, degalactosylated fetuin over repeated injections at two flow rates. It contains nine glycan and isomer features measured across 18 source observations.

Usage

glyhub_GPST000738_N_B4GalT1(
  kind = c("curated", "raw"),
  cache_dir = getOption("glyhub.cache_dir", tools::R_user_dir("glyhub", "cache")),
  refresh = FALSE,
  offline = FALSE
)

Format

A glyexp::GlycomicSE with 9 features and 18 observations. The abundance assay is in percentage within the source glycan family. rowData includes a typed glycan_structure column; features without a supported structure are NA.

Source

GlycoPOST GPST000738. Supplementary Table 1, sheets 1 and 2; composition comparisons in sheets 5 and 6; Figure S3. Study: https://doi.org/10.3389/fmolb.2026.1932584. Repository: https://glycopost.glycosmos.org/entry/GPST000738.

Arguments

kind: "curated" returns a data object; "raw" returns the path to a verified source archive. Raw archives are currently available for CPTAC.
cache_dir: Cache directory, defaulting to tools::R_user_dir("glyhub", "cache"). Set option glyhub.cache_dir to change the default.
refresh: Download the pinned resource again, even if cached.
offline: Prohibit downloads; require a verified local resource.

Details

The substrate is foetal bovine serum fetuin treated with sialidase and galactosidase. Four substrate-control columns are followed by two observations at each of 2, 4 and 8 hours at 25 microlitres/minute, and at 0, 2, 4 and 8 hours at 75 microlitres/minute. The first 25 microlitres/minute injection was excluded in the source study because of acquisition loss and poor signal.

Abundance is expressed as a percentage within the A2 or A3 glycan family, not of the total glycome. Flow and time are recorded in colData. The repeated control table is represented once; the columns do not establish biological replication or pairing.

Twelve A2/A2G1 treatment cells with duplicated, non-closing source values and twelve A3 control cells with unresolved cross-table correspondence are NA. Six additional zero measurements are NA. Original values and the separate masks remain in metadata. Summing the available values therefore need not give 100.

Nine typed compositions retain distinct source isomer labels. Four fully localized structures from Supplementary Figure S3 are encoded; ambiguous antennae and unresolved isomer mappings retain diagram evidence.

Access

Use glyhub_load("glyhub_GPST000738_N_B4GalT1") to load this dataset.