Sialylation of bovine fetuin N-glycans in an enzyme reactor

N-glycan profiles from desialylated bovine fetuin passed through an immobilised ST6GAL1 reactor. The experiment follows redistribution among sialylated forms of three precursor families during repeated injections at 25 and 75 microlitres/minute. Ten glycan features are represented across 28 source observations.

Usage

glyhub_GPST000738_N_ST6GAL1(
  kind = c("curated", "raw"),
  cache_dir = getOption("glyhub.cache_dir", tools::R_user_dir("glyhub", "cache")),
  refresh = FALSE,
  offline = FALSE
)

Format

A glyexp::GlycomicSE with 10 features and 28 observations. The abundance assay is in fraction within the source precursor glycan family. rowData includes a typed glycan_structure column; features without a supported structure are NA.

Source

GlycoPOST GPST000738. Supplementary Table 1, sheets 3 and 4; composition comparisons in sheets 7 and 8; Figure S3. Study: https://doi.org/10.3389/fmolb.2026.1932584. Repository: https://glycopost.glycosmos.org/entry/GPST000738.

Arguments

kind: "curated" returns a data object; "raw" returns the path to a verified source archive. Raw archives are currently available for CPTAC.
cache_dir: Cache directory, defaulting to tools::R_user_dir("glyhub", "cache"). Set option glyhub.cache_dir to change the default.
refresh: Download the pinned resource again, even if cached.
offline: Prohibit downloads; require a verified local resource.

Details

Seven substrate-control columns accompany seven reactor observations at 25 microlitres/minute and fourteen at 75 microlitres/minute. Injection times are 0, 2, 4 and 8 hours. The 25 microlitres/minute series has one 0-hour column and two at each later time; the 75 microlitres/minute series has four at 0, 2 and 8 hours and two at 4 hours.

Values are fractions within the H6N5, H5N5 or H5N4 precursor family. They must not be interpreted as total-glycome fractions or mixed directly with the percentage scale of the B4GalT1 dataset.

Thirteen source n.d. values are NA. Ten 25 microlitres/minute 0-hour measurements are also NA because their omission from processed sheet 7 is unexplained. An extra processed-table control lacks a raw-table identity and remains in metadata as unmatched observations. The repeated control table is represented once; biological replicate type is unresolved.

Ten typed compositions describe sialylated forms. Four fully localized Figure S3 structures are encoded; diagrams retain the evidence for unresolved positional forms.

Access

Use glyhub_load("glyhub_GPST000738_N_ST6GAL1") to load this dataset.